Porcine Circovirus Type-Ⅱ Antibody ELISA Detection Kit

Qauv TSIS MUAJ: VD510E
Abbr.:PCV-Ⅱ Ab Elisa
Qauv: serum

Xa kev nug

Hauj lwm lawm

Specification

PCV-Ⅱ Ab Elisa

Npaj siv:

Porcine Circovirus Type-Ⅱ Antibody ELISA Detection Kit yog rau kev txiav txim siab ntawm PCV antibody tshwj xeeb hauv npua cov ntshav hauv vitro. Qhov kev sim no yog siv los ntsuas cov tshuaj tiv thaiv los ntawm kev txhaj tshuaj tiv thaiv kab mob porcine circovirus hom -Ⅱ tshuaj tiv thaiv ntawm npua teb.

Qauv:ntshav qab zib

Cov ntaub ntawv muab:

Porcine Circovirus Type-Ⅱ Antibody ELISA Detection Kit

Cov txheej txheem xeem & txhais lus

KAUJ RUAM 1 Cov txheej txheem xeem

1.1 Kev npaj tshuaj ntxuav

Ua hauj lwm concentration ntxuav kua: Ua ntej siv, cov tshuaj ntxuav yuav tsum tau muab rov qab rau hauv chav tsev kub (20-25 degree) thiab shaken tusyees kom nws uniform. Tom qab ntawd dilute 20 npaug ntawm cov tshuaj ntxuav kom huv nrog dej huv (piv txwv li, 5ml cov tshuaj ntxuav tes ntxiv rau 95ml purified dej), sib tov zoo, thiab diluted ntxuav kua yuav khaws cia ntawm 2-8℃rau 7 hnub.

1.2 Kev Npaj Ua Qauv

① Tag nrho cov ntshav tsiaj raug coj mus, centrifuged ntawm 4000rpm rau 10 feeb, thiab supernatant tau sau. Serum kuj tuaj yeem coagulated ib txwm tsis muaj hemolysis.

② Dilute cov qauv 1:25 (ntxiv 5ul serum qauv rau 120ul qauv diluent) thiab sib tov kom huv si.

Nco tseg: Kev tswj tsis zoo thiab qhov zoo yuav tsum tsis txhob diluted.

1.3 Enzyme conjugate Kev Npaj

Dilute enzyme conjugate 1:25 (ntxiv 5ul enzyme conjugate rau 120ul enzyme diluent) thiab sib tov kom huv si.

1.4 Cov txheej txheem xeem

① Coj daim ntawv lo enzyme Microplate (raws li tus naj npawb ntawm cov qauv, tuaj yeem raug tshem tawm thiab siv ob peb zaug), ntxiv 100ul ntawm cov kua dej diluted rau daim ntawv lo enzyme Microplate, teem ob lub qhov dej rau qhov tsis zoo thiab cov qauv zoo, thiab ntxiv 100ul txhua qhov kev tswj tsis zoo thiab zoo rau cov tshuaj tiv thaiv zoo. Maj mam co cov qauv hauv lub qhov dej, siv lub sealing phaj zaj duab xis los npog lub phaj, thiab incubate ntawm 37℃rau 30 feeb.

② Qhib lub sealing phaj zaj duab xis, co tawm cov tshuaj nyob rau hauv lub phaj zoo, ntxiv 300µl ntawm kev ua hauj lwm concentration ntxuav kua rau txhua lub qhov dej, thiab ces muab pov tseg cov kua nyob rau hauv lub qhov dej. Rov ua cov kauj ruam saum toj no 5 zaug, thiab thaum kawg pat qhuav ntawm daim ntawv nqus dej huv.

③ Ntxiv 100µl ntawm Enzyme Conjugate rau txhua lub qhov dej, npog lub phaj, thiab incubate ntawm 37℃rau 45 feeb.

④ Qhib lub sealing phaj zaj duab xis, co tawm cov tshuaj nyob rau hauv lub phaj zoo, ntxiv 300µl ntawm kev ua hauj lwm concentration ntxuav kua rau txhua lub qhov dej, thiab ces muab pov tseg cov kua nyob rau hauv lub qhov dej. Rov ua cov kauj ruam saum toj no 5 zaug, thiab thaum kawg pat qhuav ntawm daim ntawv nqus dej huv.

⑤ Ntxiv 100µl substrate tov rau txhua qhov dej, npog lub phaj thiab incubate rau 10 feeb ntawm 37℃deb ntawm lub teeb.

⑥ Ntxiv 50µl nres tshuaj rau txhua qhov dej, thiab txiav txim siab qhov tshwm sim hauv 5 feeb.

KAUJ RUAM 2 Kev Txhais Lus

Tus nqi OD450 ntawm txhua qhov dej tau nyeem ntawm 96-zoo microplate nyeem ntawv.

2.1 Kev lees paub

Qhov nruab nrab OD450nm tus nqi ntawm kev tswj tsis zoo yog<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥5, the experimental results were valid. Otherwise, the test should be repeated.

2.2 Txoj kev suav

Rau txhua tus qauv, suav cov S/P raws li hauv qab no siv cov qauv kho thiab tswj cov nqi:

S / P=(OD_Qauv-OD_NC) / (UA_PC-OD_NC)

2.3 Cov ntsiab lus txhais

Thaum S/P<0.15, it is judged as negative.

Thaum S/P Ntau dua lossis sib npaug rau 0.25, nws raug txiav txim tias qhov zoo.

Thaum 0.15 Tsawg dua lossis sib npaug rau S / P<0.25, it is judged as suspicious, and suggest to repeat sampling and test one week later.

Cim npe nrov: porcine circovirus type-ⅱ antibody elisa detection kit, Tuam Tshoj porcine circovirus type-ⅱ antibody elisa detection kit lwm tus neeg, manufacturers, hoobkas

Tug: Anti-Ge Porcine Pseudorabies Tus kab mob Tshuaj Tiv Thaiv Kab Mob Elisa Antibody Cov khoom siv

Tom ntej: Tsis muaj