Classic Swine Fever Virus Tshuaj Tiv Thaiv Elisa Cov Khoom Siv

Kev qhia tshwj xeeb

 

 

Cov Txheej Txheem Kuaj & Kev Txhais Lus

 

Kauj ruam 1 Kuaj cov txheej txheem

1.1 Npaj Ua Tau

Working concentration washing ua kua:Ua ntej siv, kev ua tiav kev ntxuav yuav tsum tau rov qab mus txog chav sov (20-25 degree) thiab shaken tusyees los ua kom nws tsis zoo. Tom qab ntawd dilute 20 lub sij hawm lub rauv ntxuav nrog cov dej ntxuav (piv txwv, 5ml zoo, thiab siv cov kua ntxuav tawm tuaj yeem khaws cia ntawm {}} degree rau 7 hnub.

1.2 Kev Npaj Qauv Qauv

① Tag nrho cov tsiaj cov ntshav tau tshwm sim, centrifuged ntawm 4000RPM rau 10 feeb, thiab supernatant tau sau. Ntshav dej kuj tseem tuaj yeem ua rau muaj kev cuam tshuam ib txwm tsis muaj hemolysis.

② Dilute Dilute Cov Dej Daus Yuav Tsum Tau Kuaj 10 zaug hauv cov ntshav ntshiab phaj (piv txwv: Ntxiv 15

Nco tseg: tsis zoo thiab muaj txiaj ntsig kev tswj yuav tsum tsis txhob diluted.

1.3 cov txheej txheem ntsuas

①Take Tus Enzyme sau microplate (raws li tus naj npawb ntawm cov qauv, tuaj yeem tsim tawm microplate thiab siv ob peb zaug kev tswj hwm thiab kev tswj hwm zoo, thiab ntxiv 100 leej tswj tau tus kev tawm tsam zoo feem. Maj mam muab cov qauv hauv lub qhov dej, siv cov phaj sealing los npog lub phaj, thiab ncu ntawm 37 degree rau 30 feeb.

Oopen cov ntawv sib khi ua yeeb yaj kiab, co tawm ntawm kev daws hauv lub phaj zoo, ntxiv 3004L ntawm kev ua haujlwm siab ntxuav kua rau txhua qhov, thiab tom qab ntawd pov tseg cov kua hauv lub qhov dej. Rov ua cov kauj ruam saum toj no 5 zaug, thiab thaum kawg pat qhuav ntawm daim ntawv huv si.

③add 100μl ntawm enzyme conjugate rau txhua qhov zoo, npog phaj, thiab ncos ntawm 37 degree rau 30 feeb.

Oopen cov ntawv sib khi ua yeeb yaj kiab, co tawm ntawm kev daws hauv lub phaj zoo, ntxiv 3004L ntawm kev ua haujlwm siab ntxuav kua rau txhua qhov, thiab tom qab ntawd pov tseg cov kua hauv lub qhov dej. Rov ua cov kauj ruam saum toj no 5 zaug, thiab thaum kawg pat qhuav ntawm daim ntawv huv si.

⑤add 100μL Substrate daws rau txhua lub phaj thiab ncos rau 10 feeb ntawm 37 degree deb ntawm lub teeb.

⑥add 50μl nres kev daws rau txhua qhov zoo, thiab txiav txim siab qhov tshwm sim tsis pub dhau 5 feeb.

 

QEEM 2 TIM Txhais Lus

OD450 tus nqi ntawm txhua qhov dej tau nyeem nyob rau ntawm {1}}} microplate nyeem ntawv.

2.1 kev siv tau

Qhov nruab nrab Od45 0 nm muaj nuj nqis ntawm kev tswj hwm tsis zoo yog ntau dua los yog sib npaug rau 0.7, qhov kev sim tau siv tau. Txwv tsis pub, kev xeem yuav tsum rov ua dua.

2.2 Kev Xam Xyuas Xam

Rau txhua tus qauv, laij tus s / p raws li hauv qab no siv cov qauv kho thiab tswj qhov tseem ceeb:

2.3 Qhov txhais Kev Txhais Lus

Thaum s / p <{{0. 2, nws raug txiav txim vim tsis zoo.

Thaum s / p> 0. 3, Nws yog kev txiav txim ua qhov zoo.

Thaum {{{{0}}}}

 

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